Abstract
Species re-description of genus Ocypode (family: Ocypodidae) through biochemical and genetic analysis along the coast of Pakistan
Abstract
Ghost crabs are commonly found along the coastal belt of Pakistan. The review of literature revealed that no previous research has been done based on biochemical and molecular work except few preliminary taxonomic studies on genus Ocypode. Biochemical analysis carried out by using five isozyme markers Carbonate Dehydratase (CD), Catalase (CAT), Amylase (AMY), Peroxidase (PXD) and Octanol (Oct) and two general proteins by using different stain (Coomassie brilliant blue and Amido black). Among these markers CD found as a distinguishing marker to discriminate the species with significant difference (P=0.00) which deviate the HW-equilibrium. Higher level of genetic differentiation (FST=53.3%) found between two species indicating species are quite distinct from each. On molecular basis, combined data set shows that the three species (including one new species) form two highly supportive monophyletic clades with 100 bootstrap values when applied through ML, MP and BI. Genetic distance also showed higher level of variation between three species (5.5%). Ocypode rotundata showed higher level of intra-specific variation in isozyme (37% polymorphic loci) and molecular data (genetic distance = 0.7%). The three species have been confirmed by the use of four mitochondrial DNA Markers and three nuclear DNA markers. The ongoing further analysis for crabs of different localities and usage of different molecular markers includes (ITS-1 and ITS-2) likely resolve and discriminate the further more species of this genus.
Full Citation
Odhano, S., N.U. Saher, M.S. Rosenberg, and M. Kamal (2018) Species re-description of genus Ocypode (family: Ocypodidae) through biochemical and genetic analysis along the coast of Pakistan. Proceedings of Pakistan Congress of Zoology 38:416.
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